Phospho-Beclin 1-Ser15 Rabbit pAb, Unconjugated
Catalog Number:
ABB-AP1252
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| Article Name: | Phospho-Beclin 1-Ser15 Rabbit pAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP1252 |
| Supplier Catalog Number: | AP1252 |
| Alternative Catalog Number: | ABB-AP1252-100UL, ABB-AP1252-20UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | ATG6, VPS30, beclin1, Phospho-Beclin 1-Ser15 |
| This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. |
| Application Dilute: | WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Cancer,Tumor suppressors,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Autophagy,Endocrine Metabolism,Mitochondrial metabolism. |
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Western blot analysis of various lysates using Phospho-Beclin 1-Ser15 Rabbit pAb (AP1252) at 1:500 dilution. NIH/3T3 and C6 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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