Phospho-GSK3beta-S9 Mouse mAb, IgG1, Unconjugated

Product Overview

• Article Name: Phospho-GSK3beta-S9 Mouse mAb, IgG1, Unconjugated
• Biozol Catalog Number: ABB-AP1258
• Supplier Catalog Number: AP1258
• Alternative Catalog Number: ABB-AP1258-20UL,ABB-AP1258-500UL,ABB-AP1258-1000UL,ABB-AP1258-100UL
• Manufacturer: ABclonal
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: GSK3B, gsk-3beta, Phospho-GSK3beta-S9

Product Description

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.

Product Properties

• Molecular Weight: 47kDa
• Isotype: IgG1
• NCBI: 2932
• UniProt: P49841
• Purity: Affinity purification
• Target: GSK3B

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Cancer,Signal Transduction,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cell Cycle,Centrosome,Cell Cycle Control-G1 S Checkpoint,Cell Adhesion,Microtubules,Wnt -Catenin Signaling Pathway,ESC Pluripotency and Differentiation,Endocrine Metabolism,Carbohydrate metabolism,Insulin Receptor Signaling Pathway,Endocrine and metabolic diseases,Diabetes,Immunology Inflammation,B Cell Receptor Signaling Pathway,NF-kB Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Neurodegenerative Diseases Markers,Other Neurological disorders,Stem Cells,Cardiovascular,Heart,Hypertrophy.

Images

Western blot analysis of various lysates using Phospho-GSK3beta-S9 Mouse mAb (AP1258) at 1:1000 dilution. HeLa NIH/3T3 and C6 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.