Phospho-AKT1-T308+AKT2-T309+AKT3-T305 Rabbit pAb

Product Overview

• Article Name: Phospho-AKT1-T308+AKT2-T309+AKT3-T305 Rabbit pAb
• Biozol Catalog Number: ABB-AP1266
• Supplier Catalog Number: AP1266
• Alternative Catalog Number: ABB-AP1266-100UL, ABB-AP1266-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: AKT1/AKT2/AKT3, Phospho-AKT1-T308+AKT2-T309+AKT3-T305

Product Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Product Properties

• Molecular Weight: 48kDa/55kDa/51kDa/54kDa
• NCBI: 207
• UniProt: P31749
• Purity: Affinity purification
• Target: AKT

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation.

Images

Western blot analysis of lysates from Jurkat cells, using Phospho-AKT1-T308+AKT2-T309+AKT3-T305 Rabbit pAb (AP1266) at 1:1000 dilution. Jurkat cells were treated with Serum-starvation overnight at 37°C. Jurkat cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.