Phospho-FGFR3-Y724 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-FGFR3-Y724 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1274
• Supplier Catalog Number: AP1274
• Alternative Catalog Number: ABB-AP1274-20UL, ABB-AP1274-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ACH, CEK2, JTK4, CD333, HSFGFR3EX, Phospho-FGFR3-Y724

Product Description

This gene encodes a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia.

Product Properties

• Molecular Weight: 88kDa
• NCBI: 2261
• UniProt: P22607
• Purity: Affinity purification
• Target: FGFR3

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Cancer,Signal Transduction,Kinase,Tyrosine kinases,Cell Biology Developmental Biology,Growth factors,ESC Pluripotency and Differentiation,Immunology Inflammation,CDs,Stem Cells,Neural Stem Cells,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-FGFR3-Y724 Rabbit pAb (AP1274) at 1:1000 dilution. NIH/3T3 cells were treated with pervanadate (1 mM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.