Phospho-HER2/ErbB2-Y1139 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-HER2/ErbB2-Y1139 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1285
• Supplier Catalog Number: AP1285
• Alternative Catalog Number: ABB-AP1285-100UL,ABB-AP1285-20UL,ABB-AP1285-500UL,ABB-AP1285-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: NEU, NGL, HER2, TKR1, CD340, HER-2, VSCN2, MLN 19, MLN-19, c-ERB2, c-ERB-2, HER-2/neu, p185(erbB2), Phospho-HER2/ErbB2-Y1139

Product Description

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC2723]
• Molecular Weight: 138kDa
• NCBI: 2064
• UniProt: P04626
• Purity: Affinity purification
• Sequence: PEYVN
• Target: ERBB2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Cancer,Tumor immunology,Tumor-associated antigens,Tumor biomarkers,Signal Transduction,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Growth factors,Immunology Inflammation,CDs,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from A-431 cells, using Phospho-HER2/ErbB2-Y1139 (AP1285) at 1:500 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.