Phospho-Beclin 1-Ser93 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-Beclin 1-Ser93 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1287
• Supplier Catalog Number: AP1287
• Alternative Catalog Number: ABB-AP1287-20UL, ABB-AP1287-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ATG6, VPS30, beclin1, Phospho-Beclin 1-Ser93

Product Description

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Product Properties

• Molecular Weight: 52kDa
• NCBI: 8678
• UniProt: Q14457
• Purity: Affinity purification
• Target: BECN1

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Cancer,Tumor suppressors,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Autophagy,Endocrine Metabolism,Mitochondrial metabolism.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-Beclin 1-Ser93 Rabbit pAb (AP1287) at 1:500 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.