Phospho-Cytokeratin 8 (KRT8)-S74 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-Cytokeratin 8 (KRT8)-S74 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1299
• Supplier Catalog Number: AP1299
• Alternative Catalog Number: ABB-AP1299-20UL,ABB-AP1299-100UL,ABB-AP1299-1000UL,ABB-AP1299-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: K8, KO, CK8, CK-8, CYK8, K2C8, CARD2, Phospho-Cytokeratin 8 (KRT8)-S74

Product Description

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 54 kDa/56 kDa
• NCBI: 3856
• UniProt: P05787
• Purity: Affinity purification
• Sequence: LLSPL
• Target: KRT8
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Cancer,Invasion and Metastasis,Signal Transduction,Cell Biology Developmental Biology,Cell Adhesion,Cytoskeleton,Intermediate Filaments.

Images

Western blot analysis of lysates from Hep G2, using Phospho-Cytokeratin 8 (KRT8)-S74 Rabbit pAb (AP1299) at 1:1000 dilution. Hep G2 cells were treated with EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.