Phospho-POLR2A CTD- S2/S5 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-POLR2A CTD- S2/S5 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1335
• Supplier Catalog Number: AP1335
• Alternative Catalog Number: ABB-AP1335-100UL,ABB-AP1335-20UL,ABB-AP1335-500UL,ABB-AP1335-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, NEDHIB, RpIILS, hsRPB1, hRPB220, Phospho-POLR2A CTD- S2/S5

Product Description

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 217kDa
• NCBI: 5430
• UniProt: P24928
• Purity: Affinity purification
• Sequence: SYSPT
• Target: POLR2A
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,Protein phosphorylation,Neuroscience,Neurodegenerative Diseases.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-POLR2A CTD- S2/S5 Rabbit pAb (AP1335) at 1:1000 dilution. Hela cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.