Phospho-Smad2-S245/S250/S255 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-Smad2-S245/S250/S255 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1338
• Supplier Catalog Number: AP1338
• Alternative Catalog Number: ABB-AP1338-20UL,ABB-AP1338-100UL,ABB-AP1338-500UL,ABB-AP1338-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: JV18, LDS6, CHTD8, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2, Phospho-Smad2-S245/S250/S255

Product Description

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC53851]
• Molecular Weight: 52 kDa
• NCBI: 4087
• UniProt: Q15796
• Purity: Affinity purification
• Sequence: TGSPAELSPTTLSPV
• Target: SMAD2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,Cell Biology Developmental Biology,Growth factors,TGF-b-Smad Signaling Pathway,ESC Pluripotency and Differentiation,Stem Cells,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from293T cells using Phospho-Smad2-S245/S250/S255 Rabbit mAb (AP1338) at1:2000 dilution. 293T cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60 s.