Phospho-HDAC4-S632 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-HDAC4-S632 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1344
• Supplier Catalog Number: AP1344
• Alternative Catalog Number: ABB-AP1344-20UL,ABB-AP1344-100UL,ABB-AP1344-500UL,ABB-AP1344-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HD4, AHO3, BDMR, HDACA, HA6116, HDAC-4, HDAC-A, NEDCHF, NEDCHID, Phospho-HDAC4-S632

Product Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC56258]
• Molecular Weight: 119kDa
• NCBI: 9759
• UniProt: P56524
• Purity: Affinity purification
• Sequence: AQSSP
• Target: HDAC4
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Nuclear Receptor Signaling,Signal Transduction,Cell Biology Developmental Biology,Cell Cycle,Cell Cycle Control-G1 S Checkpoint,Wnt -Catenin Signaling Pathway,Immunology Inflammation,NF-kB Signaling Pathway,Stem Cells,Cardiovascular,Heart,Hypertrophy.

Images

Western blot analysis of lysates from Jurkat cells, using Phospho-HDAC4-S632 Rabbit mAb (AP1344) at1:2000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.