Phospho-IRAK4-Thr345/Ser346 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-IRAK4-Thr345/Ser346 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1351
• Supplier Catalog Number: AP1351
• Alternative Catalog Number: ABB-AP1351-1000UL,ABB-AP1351-500UL,ABB-AP1351-20UL,ABB-AP1351-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: IPD1, IMD67, REN64, IRAK-4, NY-REN-64, Phospho-IRAK4-Thr345/Ser346

Product Description

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Molecular Weight: 52kDa
• NCBI: 51135
• UniProt: Q9NWZ3
• Purity: Affinity purification
• Target: IRAK4

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Signal Transduction,Kinase,Serine threonine kinases,Immunology Inflammation,Cytokines,Interleukins,NF-kB Signaling Pathway,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling,Cardiovascular.

Images

Western blot analysis of lysates from Karpas 299 cells usingPhospho-IRAK4-Thr345/Ser346 Rabbit pAb (AP1351) at1:800 dilution. Karpas 299 cells were treated with IL-1beta (10 ng/ml) at 37°C for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:60s.