Phospho-IGF-IReceptorbeta(Tyr1135/1136)/InsulinReceptorbeta(Tyr1150/1151) Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-IGF-IReceptorbeta(Tyr1135/1136)/InsulinReceptorbeta(Tyr1150/1151) Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1352
• Supplier Catalog Number: AP1352
• Alternative Catalog Number: ABB-AP1352-100UL,ABB-AP1352-20UL,ABB-AP1352-1000UL,ABB-AP1352-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HHF5, CD220, IGFR, CD221, IGFIR, JTK13, Phospho-IGF-IReceptorbeta(Tyr1135/1136)/InsulinReceptorbeta(Tyr1150/1151)

Product Description

This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 156kDa
• NCBI: 3643
• UniProt: P06213
• Purity: Affinity purification
• Target: IGF
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-IGF-IReceptorbeta(Tyr1135/1136)/InsulinReceptorbeta(Tyr1150/1151) Rabbit pAb (AP1352) at 1:2000 dilution. HeLa cells were treated with IGF-1 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.