Phospho-PDK1/PDPK1-S241 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-PDK1/PDPK1-S241 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1357
• Supplier Catalog Number: AP1357
• Alternative Catalog Number: ABB-AP1357-100UL,ABB-AP1357-20UL,ABB-AP1357-1000UL,ABB-AP1357-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PDK1, PDPK2, PDPK2P, PRO0461, Phospho-PDK1/PDPK1-S241

Product Description

Enables 3-phosphoinositide-dependent protein kinase activity, phospholipase activator activity, and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway, regulation of protein kinase activity, and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection, cytosol, and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC57184]
• Molecular Weight: 63kDa
• NCBI: 5170
• UniProt: O15530
• Purity: Affinity purification
• Sequence: ANSFV
• Target: PDPK1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,G protein signaling,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cytoskeleton,Microfilaments,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,T Cell Receptor Signaling Pathway,NF-kB Signaling Pathway,Neuroscience.

Images

Western blot analysis of various lysates, using Phospho-PDK1/PDPK1-S241 Rabbit mAb (AP1357) at 1:2000 dilution. PC-3 cells were treated by lambda-PP mixed solution (1ul) at 30°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.