Phospho-NF-kappaB2-S866 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-NF-kappaB2-S866 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1367
• Supplier Catalog Number: AP1367
• Alternative Catalog Number: ABB-AP1367-100UL,ABB-AP1367-20UL,ABB-AP1367-1000UL,ABB-AP1367-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: p52, p100, H2TF1, LYT10, CVID10, LYT-10, NF-kB2, p49/p100, Phospho-NF-kappaB2-S866

Product Description

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC56344]
• Molecular Weight: 97kDa
• NCBI: 4791
• UniProt: Q00653
• Purity: Affinity purification
• Sequence: EDSAY
• Target: NFKB2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Jak-Stat-IL-6 Receptor Signaling Pathway,NF-kB Signaling Pathway.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-kappaB2-S866 Rabbit mAb (AP1367) at 1:500 dilution. NIH/3T3 cells were treated with TNF-alpha (20 ng/ml) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.