Phospho-Src-Y529 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-Src-Y529 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1377
• Supplier Catalog Number: AP1377
• Alternative Catalog Number: ABB-AP1377-100UL,ABB-AP1377-20UL,ABB-AP1377-1000UL,ABB-AP1377-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ASV, SRC1, THC6, c-SRC, p60-Src, Phospho-Src-Y529

Product Description

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC54665]
• Molecular Weight: 60kDa
• NCBI: 6714
• UniProt: P12931
• Purity: Affinity purification
• Target: SRC
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:2000 - 1:10000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cell Adhesion,Cytoskeleton,Microtubules,Actins,Wnt -Catenin Signaling Pathway,Immunology Inflammation,Jak-Stat-IL-6 Receptor Signaling Pathway,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit mAb (AP1377) at 1:10000 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.