Phospho-TIFA-T9 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-TIFA-T9 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1395
• Supplier Catalog Number: AP1395
• Alternative Catalog Number: ABB-AP1395-100UL,ABB-AP1395-500UL,ABB-AP1395-1000UL,ABB-AP1395-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: T2BP, T6BP, TIFAA, Phospho-TIFA-T9

Product Description

This gene encodes an adapter protein involved in adaptive and innate immunity. This protein includes a forkhead-associated (FHA) domain that specifically binds to phosphorylated serine and threonine residues. In response to bacterial infection, the encoded host cell protein undergoes an intermolecular interaction between the FHA domain and a phosphorylated threonine that leads to protein oligomerization and stimulation of the NF-kappa B and other downstream signaling pathways. This protein exhibits reduced expression in hepatocellular carcinoma and may suppress hepatocellular carcinoma progression. This protein may also play a role in the DNA damage response.

Product Properties

• Molecular Weight: 21 kDa
• NCBI: 92610
• UniProt: Q96CG3
• Purity: Affinity purification
• Sequence: ADTEE
• Target: TIFA

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Immunology Inflammation,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling.

Images

Western blot analysis of lysates from TIFA knockout (KO) 293T cells transfected with TIFA using Phospho-TIFA-T9 Rabbit pAb (AP1395) at 1:1000 dilution incubated overnight at 4°C. TIFA knockout (KO) 293T cells transfected with TIFA were treated with ADP-heptose.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.