Phospho-Myosin Light Chain 2-T18 Rabbit mAb, Unconjugated
Catalog Number:
ABB-AP1439
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| Article Name: | Phospho-Myosin Light Chain 2-T18 Rabbit mAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP1439 |
| Supplier Catalog Number: | AP1439 |
| Alternative Catalog Number: | ABB-AP1439-20UL,ABB-AP1439-100UL,ABB-AP1439-1000UL,ABB-AP1439-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | LC20, MLC2, MRLC1, MYRL2, MLC-2C, MMIHS4, Phospho-Myosin Light Chain 2-T18 |
| Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Motor Proteins,Actins,TGF-b-Smad Signaling Pathway. |
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Western blot analysis of lysates from HeLa cells, using Phospho-Myosin Light Chain 2-T18 Rabbit mAb (AP1439) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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