Phospho-RIPK1-S166 Rabbit mAb, Unconjugated
Catalog Number:
ABB-AP1448
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| Article Name: | Phospho-RIPK1-S166 Rabbit mAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP1448 |
| Supplier Catalog Number: | AP1448 |
| Alternative Catalog Number: | ABB-AP1448-20UL,ABB-AP1448-100UL,ABB-AP1448-1000UL,ABB-AP1448-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | RIP, RIP1, AIEFL, IMD57, RIP-1, Phospho-RIPK1-S166 |
| This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. |
| Application Dilute: | WB,1:2000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human, ResearchArea: Regulation of Apoptosis,Death Receptor Signaling,Inhibition of Apoptosis,NF-kappaB Signaling,p38 MAPK Activation,SAPK/JNK Signaling,Toll-like Receptor (TLR) Signaling. |
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Western blot analysis of lysates from HT-29 cells, using Phospho-RIPK1-S166 Rabbit mAb (AP1448) at 1:4000 dilution. HT-29 cells were treated with Z-VAD (50uM), TNF-alpha (50ng/ml) and BV-6 (200nM) at 37°C for 5 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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