Phospho-CRKL-Y207 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-CRKL-Y207 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1452
• Supplier Catalog Number: AP1452
• Alternative Catalog Number: ABB-AP1452-100UL,ABB-AP1452-500UL,ABB-AP1452-1000UL,ABB-AP1452-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CRKL, Phospho-CRKL-Y207

Product Description

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Product Properties

• Molecular Weight: 34kDa
• NCBI: 1399
• UniProt: P46109
• Purity: Affinity purification
• Sequence: HAYAQP
• Target: CRKL

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Protein phosphorylation,Cancer,Signal Transduction,Kinase,MAPK-JNK Signaling Pathway,Immunology Inflammation,Neuroscience.

Images

Western blot analysis of lysates from NIH/3T3 cells usingPhospho-CRKL-Y207 Rabbit pAb (AP1452) at1:500 dilution. NIH/3T3 cells were treated by lambda-PP mixed solution (1ul) at 30°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:180s.