Phospho-NF-kB p65/RelA-S529 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-NF-kB p65/RelA-S529 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1473
• Supplier Catalog Number: AP1473
• Alternative Catalog Number: ABB-AP1473-1000UL,ABB-AP1473-500UL,ABB-AP1473-20UL,ABB-AP1473-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: p65, CMCU, NFKB3, AIF3BL3, Phospho-NF-kB p65/RelA-S529

Product Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Molecular Weight: 60kDa
• NCBI: 5970
• UniProt: Q04206
• Purity: Affinity purification
• Sequence: LLSGD
• Target: RELA

Application Notes

• Application Dilute: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Endocrine Metabolism,Endocrine and metabolic diseases,Obesity,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Jak-Stat-IL-6 Receptor Signaling Pathway,NF-kB Signaling Pathway,Toll-like Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Cardiovascular.

Images

Western blot analysis of lysates from HeLa cells usingPhospho-NF-kB p65/RelA-S529 Rabbit mAb (AP1473) at1:1000 dilution. HeLa cells were treated with TNF-alpha (100 ng/ml) and Calyculin A (50 nM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 45µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:45s.