Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1496
• Supplier Catalog Number: AP1496
• Alternative Catalog Number: ABB-AP1496-1000UL,ABB-AP1496-500UL,ABB-AP1496-100UL,ABB-AP1496-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PLC1, NCKAP3, PLC-II, PLC148, PLCgamma1

Product Description

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Molecular Weight: 149kDa
• NCBI: 5335
• UniProt: P19174
• Purity: Affinity purification
• Sequence: EGS(P)FE
• Target: PLCG1

Application Notes

• Application Dilute: WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,Endocrine Metabolism,Lipid Metabolism,Immunology Inflammation,T Cell Receptor Signaling Pathway,Cardiovascular,Angiogenesis,Lipids.

Images

Western blot analysis of lysates from NIH/3T3 cells using Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit mAb (AP1496) at 1:3000 dilutionincubated overnight at 4°C. NIH/3T3 cells were treated by PDGF-BB (100 ng/ml) for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.