Phospho-VASP-S157 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-VASP-S157 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1511
• Supplier Catalog Number: AP1511
• Alternative Catalog Number: ABB-AP1511-100UL,ABB-AP1511-20UL,ABB-AP1511-1000UL,ABB-AP1511-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: VASP, Phospho-VASP-S157

Product Description

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC64201]
• Molecular Weight: 40kDa
• NCBI: 7408
• UniProt: P50552
• Purity: Affinity purification
• Sequence: RRVSNA
• Target: VASP
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Actins,Extracellular Matrix,Immunology Inflammation.

Images

Western blot analysis of lysates from HEL cells, using Phospho-VASP-S157 Rabbit mAb (AP1511) at 1:3000 dilution. HEL cells were treated with Forskolin (30 uM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.