Phospho-GSK3beta-Y216 + GSK3alpha-Y279 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-GSK3beta-Y216 + GSK3alpha-Y279 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1530
• Supplier Catalog Number: AP1530
• Alternative Catalog Number: ABB-AP1530-1000UL,ABB-AP1530-500UL,ABB-AP1530-20UL,ABB-AP1530-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: GSK3B, GSK 3 beta, GSK3A, GSK 3 alpha, Phospho-GSK3alpha-S21+GSK3beta-S9

Product Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Molecular Weight: 51kDa/46kDa
• NCBI: 2931
• UniProt: P49840
• Purity: Affinity purification
• Target: GSK3A/GSK3B

Application Notes

• Application Dilute: WB,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse, ResearchArea: Epigenetics & Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Regulation of eIF2,Cancer,Signal Transduction,Kinase,Serine/threonine kinases,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cell Cycle,Centrosome,G1/S Checkpoint,Cell Adhesion,Microtubules,Hedgehog Signaling Pathway,Wnt/beta-Catenin Signaling Pathway,ESC Pluripotency and Differentiation,Endocrine & Metabolism,Carbohydrate metabolism,Insulin Receptor Signaling Pathway,Endocrine and metabolic diseases,Diabetes,Immunology & Inflammation,B Cell Receptor Signaling Pathway,NF-kB Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Neurodegenerative Diseases Markers,Other Neurological disorders,Stem Cells,Cardiovascular,Heart,Hypertrophy,Akt downstream targets,Protein phosphorylation.

Images

Western blot analysis of lysates from NIH/3T3 cells using Phospho-GSK3beta-Y216 + GSK3alpha-Y279 Rabbit mAb (AP1530) at 1:1000 dilutionincubated overnight at 4°C. NIH/3T3 cells were treated by lambda-PP mixed solution (1µL) at 30°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.