Phospho-FAK-Y576/Y577 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-FAK-Y576/Y577 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1547
• Supplier Catalog Number: AP1547
• Alternative Catalog Number: ABB-AP1547-500UL,ABB-AP1547-20UL,ABB-AP1547-100UL,ABB-AP1547-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: FAK, FADK, FAK1, FRNK, FADK 1, PPP1R71, p125FAK, pp125FAK

Product Description

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Molecular Weight: 119kDa
• NCBI: 5747
• UniProt: Q05397
• Purity: Affinity purification
• Sequence: STYYKA
• Target: PTK2

Application Notes

• Application Dilute: WB,1:16000 - 1:64000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Tyrosine kinases,PI3K-Akt Signaling Pathway,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cytoskeleton,Actins,Extracellular Matrix,Immunology Inflammation,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from NIH/3T3 cells using Phospho-FAK-Y576/Y577 Rabbit mAb (AP1547) at 1:16000 dilutionincubated overnight at 4°C. NIH/3T3 cells were treated with pervanadate (1 mM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.