HRP-conjugated Goat anti-Mouse IgG (H+L)

Product Overview

• Article Name: HRP-conjugated Goat anti-Mouse IgG (H+L)
• Biozol Catalog Number: ABB-AS003
• Supplier Catalog Number: AS003
• Alternative Catalog Number: ABB-AS003-5X100UL, ABB-AS003-100UL, ABB-AS003-1000UL
• Manufacturer: ABclonal
• Host: Goat
• Category: Antikörper
• Application: DOT, ELISA, WB
• Species Reactivity: Mouse
• Immunogen: This information is considered to be commercially sensitive.
• Conjugation: HRP

Product Description

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Product Properties

• Purity: Affinity purification
• Target: Goat Anti-Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: WB,1:2000 - 1:10000|DB, 1:2000 - 1:10000|ELISA,1:5000 - 1:10000
• Application Notes: Cross-Reactivity: Mouse

Images

Western blot analysis of lysates from HeLa cells, using GAPDH antibody as the primary antibody.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) antibody (AS003) at 1:5000/1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.