Synthetic peptide. This information is considered to be commercially sensitive.
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Concentration:
WB
Purity:
Affinity purification
Target:
Goat Anti-Mouse Anti-Rabbit IgG H(HRP polymer)
Antibody Type:
Secondary Antibody
Application Dilute:
WB,1:500 - 1:2000
Application Notes:
Cross-Reactivity: Rabbit,Mouse
Western blot analysis of various lysates using Goat Anti-Mouse Anti-Rabbit IgG H(HRP polymer) (AS080) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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