This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Concentration:
WB
Purity:
Affinity purification
Target:
Rabbit Anti-Chicken
Antibody Type:
Secondary Antibody
Application Dilute:
WB,1:2000 - 1:20000
Application Notes:
Cross-Reactivity: Chicken
Western blot analysis of lysates from Chicken IgY Fab, using Rabbit Anti-Chicken IgY Fab (AS088) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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