Dose response curve of HRP conjugated Rabbit anti-Human IgA (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit anti-Human IgA (Fc) mAb in a 2 fold serial dilution from 1 µg/mL to 7.8*10 -3 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG4 has highly specific recognition of Human IgA (Fc) while no or minimal cross reactivity to Human IgG1,Human IgG2,Human IgG3,Human IgG4,Human IgM,Human IgE,Human IgD.
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