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Conjugation:
HRP
Concentration:
ELISA
Purity:
Affinity purification
Target:
Human Immunoglobulin lambda constant 1
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA, 1:5000-1:10000
Application Notes:
Cross-Reactivity: Human
Dose response curve of HRP conjugated Rabbit Anti-Human Lambda Light Chain mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human Lambda Light Chain mAb in a 2 fold serial dilution from 2 µg/mL to 6.1*10 -5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Human Lambda Light Chain mAb has highly specific recognition of Human IgG1, lambda,Human IgG2, lambda,Human IgG3, lambda, while no or minimal cross reactivity to Human IgG1, kappa,Human IgG2, kappa,Human IgG4, kappa,Human IgM,kappa,Rat IgG1,lambda.
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