HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb

Catalog Number: ABB-AS123
Article Name: HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb
Biozol Catalog Number: ABB-AS123
Supplier Catalog Number: AS123
Alternative Catalog Number: ABB-AS123-1000UL, ABB-AS123-100UL, ABB-AS123-5X100UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Species Reactivity: Mouse
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: HRP
Alternative Names: IgG2b, Igh-3, gamma2b
Enables antigen binding activity. Acts upstream of or within phagocytosis, positive regulation of immune response, and positive regulation of phagocytosis. Located in extracellular space. Part of immunoglobulin complex, circulating. Orthologous to several human genes including IGHG1 (immunoglobulin heavy constant gamma 1 (G1m marker)), IGHG2 (immunoglobulin heavy constant gamma 2 (G2m marker)), and IGHG3 (immunoglobulin heavy constant gamma 3 (G3m marker)).
Concentration: ELISA
Molecular Weight: 37kDa
NCBI: 16016
UniProt: P01867
Purity: Affinity purification
Target: Ighg2b
Antibody Type: Secondary Antibody
Application Dilute: ELISA, 1:5000-1:10000
Application Notes: Cross-Reactivity: Mouse, ResearchArea: Immunology,Immunoglobulins,Heavy Chain,IgG.
Dose response curve of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb in a 2 fold serial dilution from 2 µg/mL to 6.1*10 -5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Mouse IgG2b (Fc) has highly specific recognition of Mouse IgG2b while no or minimal cross reactivity to Mouse IgG1,Mouse IgG2a,Mouse IgG2c,Mouse IgG3,Mouse IgM,Mouse IgE,Mouse IgD,Rat IgG,Human IgG.