HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb

Catalog Number: ABB-AS125
Article Name: HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb
Biozol Catalog Number: ABB-AS125
Supplier Catalog Number: AS125
Alternative Catalog Number: ABB-AS125-5X100UL, ABB-AS125-100UL, ABB-AS125-1000UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: HRP
Alternative Names: IgG2
Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response, defense response to other organism, and phagocytosis. Located in extracellular space.
Concentration: ELISA
NCBI: 3501
UniProt: P01859
Purity: Affinity purification
Target: IGHG2
Antibody Type: Secondary Antibody
Application Dilute: ELISA, 1:5000-1:10000
Application Notes: Cross-Reactivity: Human(recombinant), ResearchArea: antigen binding , immunoglobulin receptor binding l , b cell receptor signaling pathway , complement activation, classical pathway , defense response to bacteria , positive regulation of B cell activation.
Dose response curve of HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb in a 2 fold serial dilution from 2 µg/mL to 6.1*10 -5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG2 (Fc) has highly specific recognition of Human IgG2,Human IgG while no or minimal cross reactivity to Human IgG1,Human IgG4,Human IgM,Rat IgG.