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The 3-fluorescence Mouse Treg Panel has been designed for identify and phenotype CD4+ T regulatory cells in Mouse blood or tissue samples. ABflo multi-fluorescence panel RK40004 covers the core CD markers of FOXP3, CD4 and CD25. Our plots showed clear subgroups of CD4+ T cells, and CD25+ FOXP3+ Treg cells (cite figures).
Concentration:
FC
Application Dilute:
FC,5 µl per 10 6 cells in 100 µl volume
Application Notes:
Cross-Reactivity: Mouse
Multicolor flow cytometry panel to identify mouse regulatory T cells. C57 BL/6 mouse splenocytes were surface-stained with ABflo 594 Rabbit anti-Mouse CD4 mAb (A24722,5 µL/Test) and ABflo 647 Rabbit anti-Mouse CD25 mAb (A23808,5 µL/Test), and then intracellularly-stained with PE Rabbit anti-Mouse FOXP3 mAb (A26227,5 µL/Test) . All antibodies are validated for Flow cytometry. CD4 T cells are defined as CD4+, Regulatory T cells are defined as CD25+ FOXP3+.
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