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The 5-fluorescence Human Activated T Cell Panel has been designed for identify and phenotype CD3+ activated T cells in Human blood or tissue samples. ABflo multi-fluorescence panel RK40006 covers the core CD markers of CD25, CD3, CD4, CD8 and CD69. Our plots showed clear subgroups of CD3+ T cells, CD4+CD3+ T cells, CD8+CD3+ T cells and CD25+CD69+ activated T cells (cite figures).
Concentration:
FC
Application Dilute:
FC,5 µl per 10 6 cells in 100 µl volume
Application Notes:
Cross-Reactivity: Human
Multicolor flow cytometry panel to identify human activated T cells. Unstimulated (left panel) and stimulated (right panel) PBMCs were surface stained with ABflo 450 Mouse anti-Human CD3 mAb (A25189,5 µL/Test), ABflo 610 Rabbit anti-Human CD4 mAb (A26218,5 µL/Test), ABflo 488 Rabbit anti-Human/Monkey CD8a mAb (A23345,5 µL/Test), PE Rabbit anti-Human CD25 mAb (A26773,5 µL/Test) and ABflo 647 Rabbit anti-Human CD69 mAb (A25892,5 µL/Test). All antibodies are validated for Flow cytometry. CD3 T lymphocytes are defined as CD3+, CD4 T cells are defined as CD4+CD3+, CD8 T cells are defined as CD8+CD3+, activated T cells are defined as CD25+CD69+.
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