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The 7-fluorescence Human TBNK Panel has been designed for reliable identification of human T, B, and NK lymphocyte populations in human blood or tissue samples.ABflo multi-fluorescence panel RK40008 covers the core CD markers of CD45, CD3, CD4, CD8, CD19, CD56 and CD16. Our plots showed clear subgroups of CD4+ lymphocytes, CD8+ T lymphocytes, CD3-CD19+ B cells, CD56brightCD16neg, CD56brightCD16dim, CD56dimCD16pos, CD56negCD16pos NK cells and CD3+CD56+ NKT cells (cite figures).
Concentration:
FC
Application Dilute:
FC,5 µl per 10 6 cells in 100 µl volume
Application Notes:
Cross-Reactivity: Human
Flow cytometry: Multicolor flow cytometry panel to identify Human TBNK cell subsets. Human PBMC were surface stained with ABflo 450 Rabbit anti-Human CD45 mAb (A25195,5 µL/Test), ABflo 488 Rabbit anti-Human CD3 mAb (A26283,5 µL/Test), ABflo 610 Rabbit anti-Human CD4 mAb (A26218,5 µL/Test), ABflo 500 Rabbit anti-Human/Monkey CD8a mAb (A26731,5 µL/Test), ABflo 700 Rabbit anti-Human/Monkey CD19 mAb (A27165,5 µL/Test), APC Rabbit anti-Human CD56/NCAM1 mAb (A26932,5 µL/Test), PE Rabbit anti-Human CD16 mAb (A24974,5 µL/Test). All antibodies are validated for Flow cytometry. CD4+ T lymphocytes are defined as CD3+CD4+, CD8+ T lymphocytes are defined as CD3+CD8+, B cells are CD3-CD19+, NK cells are defined as CD56brightCD16neg, CD56dimCD16pos, CD56negCD16pos, NKT cells are CD3+CD56+.
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