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The 4-fluorescence Human Neutrophils panel has been designed for identify and phenotype Neutrophils in Human blood or tissue samples.The 4-fluorescence Human Neutrophils panel has been designed for identify and phenotype Neutrophils in Human blood or tissue samples.ABflo multi-fluorescence panel RK40012 covers the core CD markers of CD45, CD11b, CD15/SSEA-1 and CD16. Our plots showed clear subgroups of CD45+CD15+ Granulocytes and CD11b+CD16+ Neutrophils (cite figures).
Concentration:
FC
Application Dilute:
FC,5 µl per 10 6 cells in 100 µl volume
Application Notes:
Cross-Reactivity: Human
Flow cytometry: Multicolor flow cytometry panel to identify Human Neutrophils Subsets. Human peripheral blood granulocytes were surface stained with PerCP Rabbit anti-Human CD45 mAb (A25624,5 µL/Test), ABflo 488 Mouse anti-Human CD11b mAb (A26894,5 µL/Test), PE Mouse anti-Human CD15/SSEA-1 mA (A26930,5 µL/Test), ABflo 647 Rabbit anti-Human CD16 mAb (A23400,5 µL/Test). All antibodies are validated for Flow cytometry. Granulocytes are defined as CD45+CD15+, Neutrophils are defined as CD11b+CD16+.
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