Recombinant Human Mature BMP-10 Protein

Product Overview

• Article Name: Recombinant Human Mature BMP-10 Protein
• Biozol Catalog Number: ABB-RP00771
• Supplier Catalog Number: RP00771
• Alternative Catalog Number: ABB-RP00771-20UG, ABB-RP00771-10UG, ABB-RP00771-100UG
• Manufacturer: ABclonal
• Host: Human
• Category: Proteine/Peptide
• Species Reactivity: Human
• Immunogen: Ser22-Arg424
• Alternative Names: BMP10,Bone morphogenetic protein 10, BMP-10

Product Description

BMP-10 is critical for the proper development of the heart but is not expressed until after cardiac patterning or looping are completed. BMP-10 production appears at the onset of trabeculation and chamber formation and is restricted to the right atrium in the adult heart . Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development. BMP-10 is required for the expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and the growth of embryonic cardiomyocytes. NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes. Multiple human congenital heart defects result from mutations in NKX2.5 and require BMP-10 expression . In mice, genetic knockout of ErbB leads to a similar phenotype but appears not to involve BMP-10, and knockout of the calcium channel subunit FKBP12 induces BMP-10 over-expression. BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size. BMP-10 has been shown to signal through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines . A functional BMP-10 receptor in the heart has not yet been identified, although deletion of BMPR-IA causes similar cardiac morphogenetic abnormalities. In dermal endothelial cells, BMP-10 induces migration, proliferation, and gene expression typically associated with ALK-1.

Product Properties

• Concentration: < 1 EU/µg of the protein by LAL method.
• Molecular Weight: 12.16 KDa
• NCBI: 27302
• UniProt: O95393
• Purity: 90% as determined by reducing SDS-PAGE.
• Form: Lyophilized from 0.22µm filtered solution in 4mM HCl.
• Target: BMP10

Application Notes

• Application Dilute: Lyophilized from 0.22µm filtered solution in 4mM HCl.
• Application Notes: Cross-Reactivity: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage,it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA,5% HSA,10% FBS or 5% Trehalose),and aliquot the reconstituted protein solution to minimize free-thaw cycles., ResearchArea: Cytokines & Cytokine receptors

Images

Recombinant Human Mature BMP-10 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.