Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure . In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5adesArg74 molecule . Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 . Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation . It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.
Target:
C5
Application Dilute:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.
Application Notes:
Cross-Reactivity: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage,it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA,5% HSA,10% FBS or 5% Trehalose),and aliquot the reconstituted protein solution to minimize free-thaw cycles., ResearchArea: Cytokines & Cytokine receptors
Recombinant Human Complement C5A Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
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