The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
95 % as determined by SDS-PAGE, 95 % as determined by HPLC.
Form:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 µm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Target:
SARS-CoV-2 Spike S1
Application Dilute:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 µm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Application Notes:
Cross-Reactivity: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage,it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA,5% HSA,10% FBS or 5% Trehalose),and aliquot the reconstituted protein solution to minimize free-thaw cycles., ResearchArea: SARS-CoV-2 antigens
Recombinant SARS-CoV-2 Spike S1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of SARS-COV-2 Spike S1 Protein with His tag (Cat.RP01262) was greater than 95% as determined by SEC-HPLC.
Immobilized Recombinant SARS-COV-2 Spike S1 at 2µg/mL (100 µL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.
Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 11.4 nM as determined in a SPR assay (Nicoya OpenSPR).
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