Active Recombinant Human ACE2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession Q9BYF1) fused with a 6*His,Avi tag at the C-terminus.
95 % as determined by SDS-PAGE,95 % as determined by HPLC.
Form:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 µm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Target:
ACE-2
Application Dilute:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 µm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Application Notes:
ResearchArea: SARS-CoV-2 antigens. Shipping: Ice Bag
Recombinant Human ACE-2 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC
Immobilized Human ACE2 at 2 µg/mL (100µL/well) can bind SARS-CoV-2 Spike RBD with a linear range of 0.03-4.9 ng/mL.
SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200).
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