Biotinylated Recombinant Human ACE-2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln18-Ser740) of Human ACE2 fused with a His tag and Avi tag at the C-terminal.
95 % as determined by Tris-Bis PAGE, 95 % as determined by HPLC.
Form:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.
Target:
ACE-2
Application Dilute:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.
Application Notes:
ResearchArea: SARS-CoV-2 antigens. Shipping: Ice Bag
Biotinylated Recombinant Human ACE-2 Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinlylated human ACE2 is greater than 95% as determined by SEC-HPLC.
Immobilized SARS-COV-2 S1, His Tag at 2µg/ml (100µl/Well). Dose response curve for Biotinylated Human ACE2, His Tag with EC<,sub>,50<,/sub>, of 0.24µg/ml determined by ELISA.
Immobilized SARS-COV-2 Spike RBD, His Tag at 1µg/ml (100µl/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC<,sub>,50<,/sub>, of 0.06µg/ml determined by ELISA.
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