The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
95 % as determined by SDS-PAGE, 95 % as determined by HPLC.
Form:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.
Target:
SARS-CoV-2 Spike RBD(B.1.640.2/IHU)
Application Dilute:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.
Application Notes:
Cross-Reactivity: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage,it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA,5% HSA,10% FBS or 5% Trehalose),and aliquot the reconstituted protein solution to minimize free-thaw cycles., ResearchArea: SARS-CoV-2 antigens
Recombinant SARS-CoV-2 Spike RBD(B.1.640.2/IHU) Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of SARS-Cov-2 Spike RBD (B.1.640.2/IHU) is greater than 95% as determined by SEC-HPLC.
Immobilized SARS-Cov-2 Spike RBD (B.1.640.2/IHU) , His Tag at 0.5µg/ml (100µl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC<,sub>,50<,/sub>, of 41.3ng/ml determined by ELISA.
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