Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.
95 % as determined by Tris-Bis PAGE, 95 % as determined by HPLC.
Form:
Lyophilized from a 0.2 µm filtered solution of 50mM Tris , 10 mM CaCl2,150 mM NaCl, pH 7.5. Contact us for customized product form or formulation.
Target:
MMP-8
Application Dilute:
Lyophilized from a 0.2 µm filtered solution of 50mM Tris , 10 mM CaCl2,150 mM NaCl, pH 7.5. Contact us for customized product form or formulation.
Application Notes:
Cross-Reactivity: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage,it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA,5% HSA,10% FBS or 5% Trehalose),and aliquot the reconstituted protein solution to minimize free-thaw cycles., ResearchArea: Other Recombinant Protein
Recombinant Human MMP-8 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.
Immobilized Human MMP-8, His Tag at 5µg/ml (100µl/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC<,sub>,50<,/sub>, of 3.5µg/ml determined by ELISA.
Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 µM as determined in SPR assay (Biacore T200).
Recombinant Human MMP-8 Protein cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RD,Catalog ES001). The specific activity is >,194 pmol/min/µg, as measured under the described conditions.
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