Gelsolin is a calcium-activated actin filament severing and capping protein found in many cell types and as a secreted form in the plasma of vertebrates. Gelsolin is composed of six of these domains, termed G1-6. Some reaseraches show that gelsolin can act as a transcriptional cofactor in signal transduction and its own expression and function can be influenced by epigenetic changes. The difference in the expression levels of cytoplasmic and plasma gelsolin suggests that these two different forms of gelsolin may play different roles after cardiac injury. Plasma gelsolin, as an important component of the EASS, may have evolved to rapidly clear actin filaments from the circulation that are released by injured or dead cells.
Lyophilized from a 0.22 µm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, 1mM DTT, 5% Trehalose, 0.1% Triton X-100, pH 8.0. Contact us for customized product form or formulation.
Target:
Gelsolin/GSN
Application Dilute:
Lyophilized from a 0.22 µm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, 1mM DTT, 5% Trehalose, 0.1% Triton X-100, pH 8.0. Contact us for customized product form or formulation.
Application Notes:
Cross-Reactivity: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage,it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA,5% HSA,10% FBS or 5% Trehalose),and aliquot the reconstituted protein solution to minimize free-thaw cycles., ResearchArea: Other Recombinant Protein
Recombinant Human Gelsolin/GSN Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
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