Recombinant Human Ubiquitin (K27 only) protein, E. coli
Biozol Catalog Number:
ABB-RP10070LQ
Supplier Catalog Number:
RP10070LQ
Alternative Catalog Number:
ABB-RP10070LQ-50UG, ABB-RP10070LQ-25UG
Manufacturer:
ABclonal
Host:
E. coli
Category:
Proteine/Peptide
Species Reactivity:
Human
Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K27 only), all lysine residues but lysine 27 were substituted with arginines, thereby it only supports the formation of lysine 27-linked polyubiquitin chains.
Concentration:
Please contact us for more information.
Purity:
95% as determined by reducing SDS-PAGE.
Form:
20 mM Tris, 150 mM NaCl, 2 mM betaME, 10% Glycerol
Target:
Ubiquitin (K27 only)
Application Dilute:
20 mM Tris, 150 mM NaCl, 2 mM betaME, 10% Glycerol
Application Notes:
ResearchArea: Ubiquitination related reagent
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