The recombinant anti-HEL mouse IgG1 isotype control antibody binds to neither human nor mouse samples. Recombinant mouse IgG isotype control antibodies help minimize non-specific binding of primary and capture antibodies by blocking interactions between antibody Fc fragments and Fc receptors, and preventing non-specific antibody binding to cellular proteins, carbohydrates, and lipids. Normal mouse serum and purified mouse IgGs from normal mouse serum are commonly used as non-specific passive blocking solution for antibody-based immuno-assays. Recombinant mouse IgG antibody mixes, as a direct alternative to normal mouse serum and even mouse IgGs purified from mouse serum or a non-specific passive blocking solution, can be used to eliminate interfering antibodies from immuno-assay systems using capture and detection antibodies. There is higher percentage of mouse IgG1 or IgG2b in serum from different strains of mice. Features of recombinant mouse IgG isotype control antibodies compared with normal mouse serum and serum-derived mouse IgGs: 1) Animal free production. CHO cells rather than mice are used in antibody production. 2) Lot-to-lot consistency. CHO cells which are used for antibody drug manufacturing are used to manufacture the mouse IgG isotype control antibodies in the antibody mixes. All production is under control and predictable. 3) As a targeted or passive antibody blocker to interfering antibodies like HAMA (Human Anti-Mouse Antibodies) and no binding to human tissues. In contrast, some serum-derived mouse IgGs may bind to unknown targets in some human tissues. Recombinant mouse IgG isotype control antibodies are the best normal mouse serum alternative as blocking reagents, and serum-derived mouse IgGs alternative.
Purity:
>95% Determined by SDS-PAGE
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