Fluorescent Dye 488 is a bright and photostable dye. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy. From the chemical standpoint, Fluorescent Dye 488 is a sulfonated rhodamine dye, Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated though - otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-Fluorescent Dye 488. This NHS ester is an amine reactive dye, meaning it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.
Molecular Weight:
732.74 Da
Purity:
> 80% (by 1H NMR and HPLC-MS). The balance is mostly carboxylic acid.
Form:
Dark orange solid.
Formula:
C31H32N4O13S2
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