Thiol mono-reactive Cyanine 3 dye. This reagent can be used to attach Cyanine 3 fluorophore (an analog of Cy3) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling. Labeling with Cyanine 3 maleimide is selective, and efficient. We recommend using water-soluble Sulfo-Cyanine 3 maleimide for the labeling of antibodies and other sensitive proteins.
Molecular Weight:
666.56 kDa
Purity:
95% (by 1H NMR and HPLC-MS).
Form:
Red powder.
Formula:
C36H43N4O3BF4
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