dsGreen, an analog of SYBR Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allows the use of dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen - unlabeled primers are sufficient. Unlike other preparations of dsGreen for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:. Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot. PCR tested preparation - quality guaranteed. Low fluorescence background - high fluorescence intensity gain.
Concentration:
100X
Purity:
95% (by 1H NMR, HPLC-MS, and PCR testing).
Form:
Light orange solution.
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