Fluorescein (FAM) is a popular fluorophore that has bright emission in the green area of visible spectrum. This derivative of fluorescein contains tetrazine moiety. Tetrazines react with trans-cycloalkenes and other strained olefins in a reaction called inverse electron demand Diels-Alder reaction (ied-DA). The reaction is very quick and specific. The reagent contains pure 6-isomer of FAM.
Molecular Weight:
559.53 Da
Purity:
95% (by 1H NMR and HPLC-MS).
Form:
Orange solid.
Formula:
C31H21N5O6
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