Hexachlorofluorescein (HEX) is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX-labeled oligonucleotides are used in PCR and the HEX channel is widely used in multiplex qPCR. Oligonucleotides with a HEX label can be easily generated using HEX azide and alkyne-containing oligonucleotide via azide-alkyne cycloaddition. HEX exhibits an excitation peak at 535 nm and an emission peak at 555 in green-yellow area, and it can serve as a substitute for JOE, BODIPY(TM) 530/550, VIC(TM) because of the similar spectral characteristics.
Molecular Weight:
665.09 Da
Purity:
95% (by 1H NMR and HPLC-MS).
Form:
Orange solid.
Formula:
C24H12N4Cl6O6
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